Why? Because this His 6x-tag will be used later for protein purification.
OK, let’s unpack this, starting with the pET26b plasmid. coli folA gene (and sequence of the DNA primers used in the PCR). This question is actually quite complex and requires knowledge of the DNA sequence for both pET26b plasmid and the E. If you wish to learn more about our Team Think Tanks, click here. Please check out Team Think 3: Restriction Enzyme Digests located in the Teacher Resources section.
Helping students understand how to determine DNA fragment sizes following RE digestion reactions makes for a great Team Think Tank.
Chapter 2 background – expected DNA band sizes